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Purified myoblasts were plated in collagen-coated Petri dishes and cultured in skeletal muscle cell growth medium (PromoCell Cat  C-23060) supplemented with 0.1 mg/mL primosin to protect cultures from microbial contamination, 5% fetal bovine serum, and gentamicin at 37C in a humidified atmosphere with 5% CO2. At cell isolation, all human myoblasts were at passage 4 and expanded to passage 6. Passage numbers were matched for young- and old-derived donor cells. Myoblasts from different donors were pooled in a 1:1 ratio, and aliquots were cryopreserved. 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Gene annotations were assigned using the custom annotation tables generated in-house as detailed in GL-DPPD-7110-A (<a href=\"https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable-A_1.1.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A.md\" target=\"_\" >https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable-A_1.1.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A.md</a>), with STRINGdb (version 2.16.4), PANTHER.db (version 1.0.12), and org.Hs.eg.db (version 3.19.1).","name":"GeneLab RNAseq data processing protocol","parameters":[{"@id":"#139783000650944","parameterName":{"@id":"W9tes96RPBkIuuA0eNexPPFOXF4m5lLPOIBTnVsM","annotationValue":"trimmed sequence data","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0002569","termSource":"OBI"}},{"@id":"#139783000650608","parameterName":{"@id":"hBpOurknx4YHRyk0Lle0OLdX0RSaLO8nCcSbfIVI","annotationValue":"Trimmed 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