{"hits":1,"input":"811","study":{"OSD-811":{"comments":[],"ontologySourceReferences":[{"comments":[],"description":"Experimental Factor Ontology","file":"http://data.bioontology.org/ontologies/EFO","name":"EFO","version":"132"},{"comments":[],"description":"","file":"","name":"NCIT","version":""},{"comments":[],"description":"NCI Thesaurus OBO Edition","file":"http://purl.obolibrary.org/obo/ncit.owl","name":"","version":"18.10e"},{"comments":[],"description":"Ontology for Biomedical Investigations","file":"http://data.bioontology.org/ontologies/OBI","name":"OBI","version":"29"},{"comments":[],"description":"","file":"","name":"MESH","version":""},{"comments":[],"description":"","file":"","name":"CRO","version":""}],"people":[],"publications":[],"studies":[{"assays":[{"characteristicCategories":[],"comments":[],"dataFiles":[{"@id":"#data/rawdatafile-140227417502624","comments":[],"name":"GLDS-693_rna-seq_GSM7113432_raw.fastq.gz","type":"Raw Data File"},{"@id":"#data/rawdatafile-140227417505408","comments":[],"name":"GLDS-693_rna-seq_GSM7113433_raw.fastq.gz","type":"Raw Data File"},{"@id":"#data/rawdatafile-140227417505312","comments":[],"name":"GLDS-693_rna-seq_GSM7113434_raw.fastq.gz","type":"Raw Data File"},{"@id":"#data/rawdatafile-140227417502672","comments":[],"name":"GLDS-693_rna-seq_GSM7113435_raw.fastq.gz","type":"Raw Data File"},{"@id":"#data/rawdatafile-140227417503488","comments":[],"name":"GLDS-693_rna-seq_GSM7113436_raw.fastq.gz","type":"Raw Data File"},{"@id":"#data/rawdatafile-140227417502096","comments":[],"name":"GLDS-693_rna-seq_GSM7113437_raw.fastq.gz","type":"Raw Data File"}],"filename":"a_OSD-811_transcription-profiling_rna-sequencing-(rna-seq)_Illumina.txt","materials":{"otherMaterials":[{"@id":"#material/extract-140227417503248","characteristics":[],"name":"GSM7113432","type":"Extract Name"},{"@id":"#material/extract-140227417501856","characteristics":[],"name":"GSM7113433","type":"Extract Name"},{"@id":"#material/extract-140227417503920","characteristics":[],"name":"GSM7113434","type":"Extract Name"},{"@id":"#material/extract-140227417503392","characteristics":[],"name":"GSM7113435","type":"Extract Name"},{"@id":"#material/extract-140227417502768","characteristics":[],"name":"GSM7113436","type":"Extract Name"},{"@id":"#material/extract-140227417502432","characteristics":[],"name":"GSM7113437","type":"Extract Name"}],"samples":[{"@id":"#sample/140227415249488","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113432"},{"@id":"#sample/140227415249392","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113433"},{"@id":"#sample/140227415250640","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113434"},{"@id":"#sample/140227415249104","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113435"},{"@id":"#sample/140227415251936","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113436"},{"@id":"#sample/140227415249248","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113437"}],"sources":[]},"measurementType":{"@id":"jGo9iA3xprysX2IZmhNYRdsyHQEGg4GuLcAiq5h4","annotationValue":"transcription profiling","comments":[],"termAccession":"obo:OBI_0000424","termSource":"OBI"},"processSequence":[{"@id":"#process/140227417502528","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415249488"}],"nextProcess":{"@id":"#process/140227417503632"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227401849392","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415249392"}],"nextProcess":{"@id":"#process/140227422272480"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227401847280","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415250640"}],"nextProcess":{"@id":"#process/140227399383408"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227401847040","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415249104"}],"nextProcess":{"@id":"#process/140227417504976"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227422269984","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415251936"}],"nextProcess":{"@id":"#process/140227415852320"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227422272432","comments":[],"date":"","executesProtocol":{"@id":"#140227396526960"},"inputs":[{"@id":"#sample/140227415249248"}],"nextProcess":{"@id":"#process/140227415852128"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227407173184"},"value":"Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip"}],"performer":""},{"@id":"#process/140227417503632","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417503248"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417502624"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227417502528"}},{"@id":"#process/140227422272480","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417501856"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417505408"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227401849392"}},{"@id":"#process/140227399383408","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417503920"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417505312"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227401847280"}},{"@id":"#process/140227417504976","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417503392"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417502672"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227401847040"}},{"@id":"#process/140227415852320","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417502768"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417503488"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227422269984"}},{"@id":"#process/140227415852128","comments":[],"date":"","executesProtocol":{"@id":"#140227396526672"},"inputs":[{"@id":"#material/extract-140227417502432"}],"nextProcess":{"@id":"#process/140227415853040"},"outputs":[{"@id":"#data/rawdatafile-140227417502096"}],"parameterValues":[{"category":{"@id":"#140227416115616"},"value":"polyA enrichment"},{"category":{"@id":"#140227416116576"},"value":"SINGLE"},{"category":{"@id":"#140227416114800"},"value":"UNSTRANDED"},{"category":{"@id":"#140227416116480"},"value":{"@id":"K03xWTy3bHjYD12yIk8rq7IoBxIRPiwExdyMUUBC","annotationValue":"Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio)","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"performer":"","previousProcess":{"@id":"#process/140227422272432"}},{"@id":"#process/140227415853040","comments":[],"date":"","executesProtocol":{"@id":"#140227396527296"},"inputs":[],"nextProcess":{"@id":"#process/140227422458832"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416114992"},"value":"Illumina NovaSeq 6000"}],"performer":"","previousProcess":{"@id":"#process/140227415852128"}},{"@id":"#process/140227415852800","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417502624"}],"nextProcess":{"@id":"#process/140227422458160"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"11723763"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.68"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227415852512","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417505408"}],"nextProcess":{"@id":"#process/140227422459360"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"52726642"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.83"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227415853760","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417505312"}],"nextProcess":{"@id":"#process/140227422460272"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"20860986"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.70"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227415853424","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417502672"}],"nextProcess":{"@id":"#process/140227422458256"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"20085856"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.44"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227422458304","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417503488"}],"nextProcess":{"@id":"#process/140227394687808"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"53160010"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.67"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227422458832","comments":[],"date":"","executesProtocol":{"@id":"#140227407172320"},"inputs":[{"@id":"#data/rawdatafile-140227417502096"}],"nextProcess":{"@id":"#process/140227394687136"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227416117152"},"unit":{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg"},"value":"44905010"},{"category":{"@id":"#140227416116000"},"unit":{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe"},"value":"0.62"},{"category":{"@id":"#140227416113552"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_raw.fastq.gz"},{"category":{"@id":"#140227416115184"},"value":"GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_raw_multiqc_GLbulkRNAseq.html"}],"performer":"","previousProcess":{"@id":"#process/140227415853040"}},{"@id":"#process/140227422458160","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113432_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227415852800"}},{"@id":"#process/140227422459360","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113433_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227415852512"}},{"@id":"#process/140227422460272","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113434_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227415853760"}},{"@id":"#process/140227422458256","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113435_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227415853424"}},{"@id":"#process/140227394687808","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113436_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227422458304"}},{"@id":"#process/140227394687136","comments":[],"date":"","executesProtocol":{"@id":"#140227407173856"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227422491216"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_trimmed.fastq.gz"},{"category":{"@id":"#140227422491168"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_trimming_report.txt"},{"category":{"@id":"#140227422494336"},"value":"GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_trimmed_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491648"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam,GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_Aligned.sortedByCoord_sorted.out.bam.bai,GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_Aligned.toTranscriptome.out.bam,GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_SJ.out.tab"},{"category":{"@id":"#140227422494144"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_unmapped.fastq.gz"},{"category":{"@id":"#140227422492704"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_Log.final.out"},{"category":{"@id":"#140227422494288"},"value":"GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_align_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422491792"},"value":"GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_geneBody_cov_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_infer_exp_multiqc_GLbulkRNAseq.html,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_read_dist_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422494480"},"value":"GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq.genes.results,GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq.isoforms.results,GLDS-693_rna_seq_GSM7113437_GLbulkRNAseq_rRNArm.genes.results"},{"category":{"@id":"#140227422491936"},"value":"GLDS-693_rna_seq_RSEM_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_STAR_Unnormalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_RSEM_Unnormalized_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492368"},"value":"GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq_data.zip,GLDS-693_rna_seq_RSEM_count_multiqc_GLbulkRNAseq.html"},{"category":{"@id":"#140227422492800"},"value":"GLDS-693_rna_seq_Normalized_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_GLbulkRNAseq.csv,GLDS-693_rna_seq_Normalized_Counts_rRNArm_GLbulkRNAseq.csv,GLDS-693_rna_seq_VST_Counts_rRNArm_GLbulkRNAseq.csv"},{"category":{"@id":"#140227422492128"},"value":"GLDS-693_rna_seq_SampleTable_GLbulkRNAseq.csv,GLDS-693_rna_seq_contrasts_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_GLbulkRNAseq.csv,GLDS-693_rna_seq_differential_expression_rRNArm_GLbulkRNAseq.csv"}],"performer":"","previousProcess":{"@id":"#process/140227422458832"}}],"technologyPlatform":"Illumina","technologyType":{"@id":"FGPgUG40RqNJgQ76DppeJCp32q6pDjuTqpVn4qL3","annotationValue":"RNA Sequencing (RNA-Seq)","comments":[],"termAccession":"obo:OBI_0001271","termSource":"OBI"},"unitCategories":[{"@id":"QzsdOSD19kEspoFDZ7FXPfWP017I6qBWqwvSvjPe","annotationValue":"percent","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000187","termSource":""},{"@id":"wlRETTZuaJv8SbT4KZfCNYaSK5G9d7CuW5MGgCrg","annotationValue":"read","comments":[],"termAccession":"http://purl.obolibrary.org/obo/SO_0000150","termSource":""}]}],"characteristicCategories":[{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN","characteristicType":{"@id":"Ks6c57ph9zHq3qalQ5qd4HlmInrXBkjIZDPyauq5","annotationValue":"Cell Culture","comments":[],"termAccession":"","termSource":""}},{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30","characteristicType":{"@id":"LHmZyms6bEdkUL5QaHV42kuzI1ImB8hJYAjcA0Wu","annotationValue":"Material Type","comments":[],"termAccession":"","termSource":""}},{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi","characteristicType":{"@id":"mlZb2gHEWcShWNCdbe4vOMc8yyux2QmLr9Ip5N4s","annotationValue":"Organism","comments":[],"termAccession":"","termSource":""}},{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu","characteristicType":{"@id":"Zopv1K1kpeenmFoUBVOq22MXwMWtDP0GGGErxpQn","annotationValue":"cell line","comments":[],"termAccession":"","termSource":""}},{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA","characteristicType":{"@id":"Cp0sFLsqfKUfk0YyXq7uhPWVxqTxjqKwNollzOnk","annotationValue":"cell type","comments":[],"termAccession":"","termSource":""}}],"comments":[{"name":"Acknowledgments","value":"We would like to thank astronauts Jessica Meir and Andrew Morgan for performing the cell culture experiments aboard the International Space Station. We also thank Kathryn Pellegrini and Gregory Tharp at the Yerkes Genomics Core of Emory University for RNA sequencing and data deposit. A special thanks to Dr. Bill McLamb and Dr. Marc Giulianotti of the CASIS for their guidance during this project."},{"name":"DOI","value":"10.26030/xkj0-3s54"},{"name":"Data Source Accession","value":"GSE228063"},{"name":"Data Source Link","value":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE228063"},{"name":"Experiment Platform","value":"Multi-use Variable-gravity Platform (MVP)"},{"name":"Flight Program","value":"International Space Station (ISS)"},{"name":"Funding","value":"This study was supported in part by grants from the Center for Advancement of Science in Space (GA-2017-266 and GA-2023-9022), the National Institutes of Health (R01HL136345 and R01AA028527), and the National Science Foundation (CBET 1926387)."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":""},{"name":"Mission End","value":"04/07/2020"},{"name":"Mission Link","value":"https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-20"},{"name":"Mission Name","value":"SpaceX-20"},{"name":"Mission Start","value":"03/07/2020"},{"name":"Project Identifier","value":"MVP2-Cell-03"},{"name":"Project Link","value":""},{"name":"Project Title","value":""},{"name":"Project Type","value":"Spaceflight Study"},{"name":"Space Program","value":"CASS, NIH, NSF"},{"name":"Study Grant Number","value":""}],"description":"Efficient generation of cardiomyocytes from human-induced pluripotent stem cells (hiPSCs) is important for their application in basic and translational studies. Space microgravity can significantly change cell activities and function. Previously, we reported upregulation of genes associated with cardiac proliferation in cardiac progenitors derived from hiPSCs that were exposed to space microgravity for 3 days. Here we investigated the effect of long-term exposure of hiPSC-cardiac progenitors to space microgravity on global gene expression. Cryopreserved 3D hiPSC-cardiac progenitors were sent to the International Space Station (ISS) and cultured for 3 weeks under ISS microgravity and ISS 1\u2009G conditions. RNA-sequencing analyses revealed upregulation of genes associated with cardiac differentiation, proliferation, and cardiac structure/function and downregulation of genes associated with extracellular matrix regulation in the ISS microgravity cultures compared with the ISS 1\u2009G cultures. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes mapping identified the upregulation of biological processes, molecular function, cellular components, and pathways associated with cell cycle, cardiac differentiation, and cardiac function. Taking together, these results suggest that space microgravity has a beneficial effect on the differentiation and growth of cardiac progenitors.","factors":[{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k","comments":[],"factorName":"Spaceflight","factorType":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2","comments":[],"factorName":"Altered Gravity","factorType":{"@id":"PDmY1sKTXFdQ1hZVG6o5TrZ6BxH0kq1JMb1RuJZC","annotationValue":"Gravity, Altered","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D018470","termSource":"MESH"}}],"identifier":"OSD-811","materials":{"otherMaterials":[],"samples":[{"@id":"#sample/140227415249488","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113432"},{"@id":"#sample/140227415249392","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113433"},{"@id":"#sample/140227415250640","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"5805IR5cmmoAkFwij0EqZpCbW8AFUD3XIwslpKqD","annotationValue":"uG","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}}],"name":"GSM7113434"},{"@id":"#sample/140227415249104","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113435"},{"@id":"#sample/140227415251936","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113436"},{"@id":"#sample/140227415249248","characteristics":[{"category":{"@id":"399SzhBQzExSjIr4pjc31bssabQKEbyJxfwbrlYi"},"value":{"@id":"RjTzuANoXpxSw3S8Ja6Sw5fzEzdeM5I3f94uEHCA","annotationValue":"Homo sapiens","comments":[],"termAccession":"http://purl.bioontology.org/ontology/NCBITAXON/9606","termSource":""}},{"category":{"@id":"49juZ4FGB57I9ooIymVnW2cIJgBOgOHjamafkg30"},"value":{"@id":"UEVzqbPKmM0yV9c3akyq7CMsiR4NBgHX4zFDdsLa","annotationValue":"Cells, Cultured","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D002478","termSource":"MESH"}},{"category":{"@id":"Y8Jag43Gwz0ORZdEinEkxnPSPlHCBl1vBUIGfjpu"},"value":{"@id":"lHX5QQLrFBK3ffTz97gxzlA8J1Gs1ItGhGYSL5vJ","annotationValue":"IMR90 hiPSC","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"category":{"@id":"dFCQ68SfXpZ2u9e3OnamUgPmR3uxAK3EOPor5wKA"},"value":{"@id":"rmAeaGoJ7qA8KF8zEisMIBUu6DpT9bHCMB7RnCgT","annotationValue":"Myocytes, Cardiac","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D032383","termSource":"MESH"}},{"category":{"@id":"lyHPZJac9jCAhFkLJ5RpLjFC3stTfMCcRWZbBGFN"},"value":{"@id":"hwl0PpTrOnquUczAPRi7DlTvtgpaTOBb4ydQmmJ7","annotationValue":"Induced Pluripotent Stem Cells","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D057026","termSource":"MESH"}}],"derivesFrom":[],"factorValues":[{"@id":"","category":{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k"},"value":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"","category":{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2"},"value":{"@id":"PaQgTF5Y1X4uTbLGLxOb2RpmoxZnW0gzeO0BeCR1","annotationValue":"1G by centrifugation","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"name":"GSM7113437"}],"sources":[{"@id":"#source/140227420807520","characteristics":[],"name":"s001_IMR90ISS \u00b5G"},{"@id":"#source/140227415250112","characteristics":[],"name":"s002_IMR90ISS \u00b5G"},{"@id":"#source/140227415250448","characteristics":[],"name":"s003_IMR90ISS \u00b5G"},{"@id":"#source/140227415248960","characteristics":[],"name":"s004_IMR90ISS 1G"},{"@id":"#source/140227415251024","characteristics":[],"name":"s005_IMR90ISS 1G"},{"@id":"#source/140227415252704","characteristics":[],"name":"s006_IMR90ISS 1G"}]},"people":[{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Hyun","lastName":"Hwang","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Antonio","lastName":"Rampoldi","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Parvin","lastName":"Forghani","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Dong","lastName":"Li","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Jordan","lastName":"Fite","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Gene","lastName":"Boland","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"","affiliation":"","comments":[],"email":"","fax":"","firstName":"Kevin","lastName":"Maher","midInitials":"","phone":"","roles":[{"@id":"asP5P3FTC2GPYBrPl2fdDgnpPyUkTQ5ZAS42LmrD","annotationValue":"Author","comments":[],"termAccession":"http://purl.obolibrary.org/obo/NCIT_C42781","termSource":""}]},{"@id":"","address":"Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta, Atlanta, GA, USA","affiliation":"Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta, Atlanta, GA, USA","comments":[],"email":"chunhui.xu@emory.edu","fax":"","firstName":"Chunhui","lastName":"Xu","midInitials":"","phone":"","roles":[{"@id":"HJPRq6x6GhRrWvebUJbKqTYWJMttZH85UbFKx2NQ","annotationValue":"conceptualization role","comments":[],"termAccession":"http://purl.org/credit/ontology","termSource":"CRO"}]}],"processSequence":[{"@id":"#process/140227415252320","comments":[],"date":"","executesProtocol":{"@id":"#140227416556832"},"inputs":[{"@id":"#sample/140227415249488"},{"@id":"#sample/140227415249392"},{"@id":"#sample/140227415250640"},{"@id":"#sample/140227415249104"},{"@id":"#sample/140227415251936"},{"@id":"#sample/140227415249248"}],"nextProcess":{"@id":"#process/140227415250400"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227422493712"},"unit":{"@id":"s4mQc0cEaxmkRxH4swt1u3Kwmsdo3hiNE43z9pX9"},"value":"24"},{"category":{"@id":"#140227422492464"},"value":{"@id":"fK4LaYrWQJnaSMxYDSwgFStInFoOCS1e7nwgyONU","annotationValue":"Aggrewell 400 plates (STEMCELL Technologies)","comments":[],"termAccession":"","termSource":""}}],"performer":""},{"@id":"#process/140227415250400","comments":[],"date":"","executesProtocol":{"@id":"#140227416555824"},"inputs":[],"nextProcess":{"@id":"#process/140227399421040"},"outputs":[],"parameterValues":[{"category":{"@id":"#140227396526288"},"unit":{"@id":"ixhZXi79C7bGPbpBgn0nhYS3WcKUHFxvfrXat1ju"},"value":"37"},{"category":{"@id":"#140227396528880"},"value":{"@id":"kdX3c9Ntod13fi0QSQlofMZFeEXS73UhvG6Dl18J","annotationValue":"Suspension Culture","comments":[],"termAccession":"http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C20217","termSource":"NCIT"}},{"category":{"@id":"#140227396529024"},"value":{"@id":"KOIabVHER5xsCEYcDKIKPQE00uO9LTkUvCQGsUTN","annotationValue":"CO2-independent medium with 10\u2009uM ROCK inhibitor","comments":[],"termAccession":"","termSource":""}},{"category":{"@id":"#140227396528256"},"value":{"@id":"cOT9Au7y1XfwPM8fzHB9JHzEi2Ke7gBjRitL36Pe","annotationValue":"Multi-use Variable-gravity Platform (MVP)","comments":[],"termAccession":"","termSource":""}},{"category":{"@id":"#140227396527680"},"unit":{"@id":"0egjOe4tupLenTJ2RAdBhRffOpl8xZWv0FRLbF9W"},"value":"22"},{"category":{"@id":"#140227396529552"},"unit":{"@id":"0egjOe4tupLenTJ2RAdBhRffOpl8xZWv0FRLbF9W"},"value":"25"}],"performer":"","previousProcess":{"@id":"#process/140227415252320"}},{"@id":"#process/140227399421040","comments":[{"name":"Experiment Start Date (DD-Mon-YYYY)","value":"14-Mar-2020"},{"name":"Mission Return Date (DD-Mon-YYYY)","value":"07-Apr-2020"}],"date":"","executesProtocol":{"@id":"#140227416558176"},"inputs":[],"outputs":[],"parameterValues":[{"category":{"@id":"#140227407174528"},"value":{"@id":"Eh8kGKU81JaCgCOjoF7sz233pBxrznhGAmRZvwd7","annotationValue":"Fresh","comments":[],"termAccession":"http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#C75531","termSource":"NCIT"}},{"category":{"@id":"#140227407175008"},"value":""}],"performer":"","previousProcess":{"@id":"#process/140227415250400"}}],"protocols":[{"@id":"#140227416556832","comments":[],"components":[],"description":"Cell culture and cryopreservation of cardiac progenitor spheres\nFor the spaceflight experiment, we prepared cryopreserved cardiac progenitor spheres from IMR90 hiPSC lines (WiCell Research Institute) that were cultured in a feeder-free condition on Matrigel-coated plates and daily fed with mTeSR1 medium. For the induction of IMR90 cardiomyocyte differentiation, hiPSCs were treated with growth factors. Briefly, when compact colonies reached greater than 95% confluence, cells were treated with 100\u2009ng/mL activin A from differentiation day 0 till day 1 and then 10\u2009ng/mL bone morphogenic protein-4 (BMP4) from day 1 till day 4 in RPMI/B27 insulin-free medium (RPMI 1640 with 2% B27 minus insulin). Cells were maintained in RPMI/B27 medium (RPMI 1640 with 2% B27 supplement with insulin) until differentiation day 6 when cardiac progenitor spheres were generated using microscale tissue engineering using Aggrewell 400 plates (STEMCELL Technologies). Briefly, cells were dissociated using 0.25% trypsin-EDTA (Thermo Fisher Scientific), resuspended for cell counting, then seeded into the Aggrewell 400 plates at a concentration of 1.8\u2009\u00d7\u200910^6 cells/well (1500 cells/microwell). Cells were cultured in RPMI/B27 medium and 10\u2009uM ROCK inhibitor Y-27632 to facilitate cell survival following dissociation. After 24\u2009h, cardiac spheres were cryopreserved.\n\nFor cryopreservation, cardiac progenitor spheres were resuspended in 0.5\u2009mL cryopreservation medium (90% fetal bovine serum and 10% dimethyl sulfoxide with 10\u2009uM ROCK inhibitor) and transferred into cryosyringes at 0.5\u2009mL/cryosyringe. Cardiac spheres were initially pre-cooled at 4\u2009C for up to 25\u2009min to maximize cryopreservation efficiency and then stored at \u221280\u2009C in a cooling box.","name":"Growth Protocol","parameters":[{"@id":"#140227422493712","parameterName":{"@id":"mkMLgsYsxmGca6ddOMTX48lEwhAqyQxOSB7vdVCa","annotationValue":"Pre-treatment Growth Duration","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422492464","parameterName":{"@id":"5XdcP8MBaCf6NJTAqpXynw12CkkFctKZAPd2iR2R","annotationValue":"Pre-treatment Culture Vessel","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"0gMEkcMNeDUnXJOXyMiO3HWs7cSThiG9RpoYliDD","annotationValue":"growth protocol","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0003789","termSource":"EFO"},"uri":"","version":""},{"@id":"#140227416555824","comments":[],"components":[],"description":"ISS cell culture operation\nFor thawing cells on the ISS, cryosyringes containing cardiac progenitor spheres were placed in a thermoblock at 37\u2009C for 5\u2009min. The cells were then injected into cell culture chambers of the MVP modules containing a CO2-independent medium with 10\u2009uM ROCK inhibitor. The MVP modules were re-installed into the MVP facility, which started with a medium flush cycle to replace the medium with a new culture medium (20\u2009mL per chamber; ~2\u00d7 chamber volume), to flush out the DMSO in the cryopreserved cell solution (0.5\u2009mL/cryosyringe). The cells were cultured at 37\u2009C in the MVP system for 22 days on the ISS with medium exchange every other day.","name":"Treatment protocol","parameters":[{"@id":"#140227396529552","parameterName":{"@id":"ZjmSSbPuCF9KW9S9qrQGc9mjnHs9TzHb1bMgnKcX","annotationValue":"duration","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0000433","termSource":"EFO"}},{"@id":"#140227396526288","parameterName":{"@id":"a63OGcE6Sw5TMHUrfgVAO7JTv9cpAPPOCK9gyUcF","annotationValue":"Growth Temperature","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227396528880","parameterName":{"@id":"3uPhmGzj0YEXYcPcGadgQsEsE0nL0jOiU714v5gT","annotationValue":"Growth Property","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227396529024","parameterName":{"@id":"KYfj5W8EUWSlT51HcJp7uPOjZ1ZBFefKS1y2EkvO","annotationValue":"Growth Medium","comments":[],"termAccession":"http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl","termSource":"NCIT"}},{"@id":"#140227396528256","parameterName":{"@id":"GwIb4Ediivl3DNjtb6prhRbAMoq8WzGwbBOMpLFC","annotationValue":"Culture Vessel","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227396527680","parameterName":{"@id":"B3r6hP0kLpYHlt4aLvxq0MaY3s85E7YLcPuhak4F","annotationValue":"Growth Duration","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"PGCRAeZJDifKUY4b2TtcTGR2iEqwo3p6PKTVbSlo","annotationValue":"sample treatment protocol","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0003809","termSource":"EFO"},"uri":"","version":""},{"@id":"#140227416558176","comments":[],"components":[],"description":"At the end of the mission, live cultures were returned to the ground via warm storage after having been cultured aboard the ISS. Upon arrival at Emory University, cardiac spheres were transferred immediately into an incubator and let recover overnight. The following day cardiac spheres were transferred from the collection bags into low adhesion dishes. Medium was changed from CO2-independent medium to standard cardiomyocyte culture medium (RPMI/B27 medium), and cardiac spheres were then maintained overnight, followed by RNA isolation.","name":"sample collection","parameters":[{"@id":"#140227407174528","parameterName":{"@id":"eUm691aR7an5kII9bYi7JOSQEcghOzzbVfEjAtOG","annotationValue":"Sample Preservation Method","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}},{"@id":"#140227407175008","parameterName":{"@id":"vbrzINCaNhslTc1WulmcigpF6afqM7x3H1PCAdm0","annotationValue":"Sample Storage Temperature","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}}],"protocolType":{"@id":"zaKRMPLXOBudyE1ROMNBjnZXViwkiB6PT7wea0yd","annotationValue":"sample collection","comments":[],"termAccession":"","termSource":""},"uri":"","version":""},{"@id":"#140227396526960","comments":[],"components":[],"description":"RNA was isolated using RNeasy Mini Kit (Qiagen) as per the manufacturer\u2019s instructions. RNA concentration was measured using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). RNA sequencing, quality control, and transcriptome mapping were done by the Yerkes National Primate Research Center of Emory University. Total RNA quality was tested using an Agilent 4200 TapeStation and RNA 6000 Nano and Pico Chip (Agilent Technologies). RNA samples of triplicate ISS uG and ISS 1G cultures collected after long-term exposure to space microgravity were subjected to library preparation and sequencing.","name":"Nucleic Acid Extraction","parameters":[{"@id":"#140227407173184","parameterName":{"@id":"Hop5C9AJDN9g3VYqtIJtB2A611oQjKFpzG6shAoZ","annotationValue":"QA Instrument","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"hvmnimkOSUuDnRXoMCmOVO622eV9H3rhB5Orz2Zc","annotationValue":"Nucleic Acid Extraction","comments":[],"termAccession":"","termSource":""},"uri":"","version":""},{"@id":"#140227396526672","comments":[],"components":[],"description":"Two nanograms of total RNA were used as input for cDNA synthesis, using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer\u2019s instructions. Amplified cDNA was fragmented and appended with dual-indexed bar codes using the NexteraXT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation. Libraries were pooled at equimolar concentrations.","name":"Library Construction","parameters":[{"@id":"#140227416115616","parameterName":{"@id":"SXpkstgzgf9WOhn8BRUImqf5kbHf1TJ3GjkLiaMg","annotationValue":"Library Selection","comments":[],"termAccession":"","termSource":""}},{"@id":"#140227416116576","parameterName":{"@id":"rlzTK2chbZkUcaCWHWpzFmlVUzKNZ7DXWAzUXMTb","annotationValue":"Library Layout","comments":[],"termAccession":"","termSource":""}},{"@id":"#140227416114800","parameterName":{"@id":"FgvdSWC3uI17LsXvErGgSgUHhsklR2Ok3RnEeJgE","annotationValue":"Stranded","comments":[],"termAccession":"","termSource":""}},{"@id":"#140227416116480","parameterName":{"@id":"8WYoCyz2C7EHCUeJopWqPTSkwz6WD98uPi77l4Ik","annotationValue":"Library Kit","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"jlgnONtnl1cbnUwKKehnj9aCWkaf8ZPvhHTdzhV8","annotationValue":"Library Construction","comments":[],"termAccession":"","termSource":""},"uri":"","version":""},{"@id":"#140227396527296","comments":[],"components":[],"description":"Library pools were sequenced on an Illumina NovaSeq 6000 at 100SR, yielding an average of 30 million reads per sample.","name":"Nucleic Acid Sequencing","parameters":[{"@id":"#140227416114992","parameterName":{"@id":"QZv3O6FfjrX7Rj1AdVBpE7MMkvX0gDPFNyhTgsul","annotationValue":"Sequencing Instrument","comments":[],"termAccession":"","termSource":""}},{"@id":"#140227416117200","parameterName":{"@id":"DOWz9RyvfMoltYGyC7An2WqtHdVXvLLJU32JiRbM","annotationValue":"Read Length","comments":[],"termAccession":"http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl","termSource":"NCIT"}},{"@id":"#140227416113312","parameterName":{"@id":"IYKxiQ3B9zomxnR3I4FMKo0N5J99QeXhWoF6Vpzl","annotationValue":"Base Caller","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"vbHeywu5lMpfsh3jNyY18mRWy5aZupc8gVSXD5Uh","annotationValue":"Nucleic Acid Sequencing","comments":[],"termAccession":"","termSource":""},"uri":"","version":""},{"@id":"#140227407172320","comments":[],"components":[],"description":"Fastq format was checked with fastq_utils v0.25.1. Quality assessment of reads was performed with FastQC v0.12.1 and reports were combined with MultiQC v1.26. rRNA percentages of reads were estimated with the 'hts_SeqScreener' program of htstream v1.3.3 screened against a fasta file of H-sapiens rRNA sequences retrieved from NCBI on 13-May-2024.","name":"GeneLab raw data processing protocol","parameters":[{"@id":"#140227416117152","parameterName":{"@id":"Ohs1BbENFJo4CWnwszKmpZ4g1nZJNqqJv60k0wyP","annotationValue":"Read Depth","comments":[],"termAccession":"http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl","termSource":"NCIT"}},{"@id":"#140227416116000","parameterName":{"@id":"RaWNd099ZqS9NO9TqfvpU9arANv1Do2iAJueQJMm","annotationValue":"rRNA Contamination","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227416115184","parameterName":{"@id":"WoqQnOe0wYp5oJdMtNlLbe7bcXgG6P1BgtOCncYd","annotationValue":"MultiQC File Names","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227416113552","parameterName":{"@id":"XGdRyLdFBrQ5sINQ7N8WhowWQEznWY4WnzAmBL6l","annotationValue":"Merged Sequence Data","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"gHRCpX8ZCZrvMb1jv0PpSihU4DurynDUUnkOSfet","annotationValue":"data transformation","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0200000","termSource":"OBI"},"uri":"","version":""},{"@id":"#140227407173856","comments":[],"components":[],"description":"Data were processed as described in GL-DPPD-7101-G (<a href=\"https://github.com/nasa/GeneLab_Data_Processing/blob/master/RNAseq/Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md\" target=\"_\" >https://github.com/nasa/GeneLab_Data_Processing/blob/master/RNAseq/Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md</a>), using NF_RCP version 2.1.0 (https://github.com/nasa/GeneLab_RNASeq_Workflow/tree/NF_RCP_2.1.0). In short, raw fastq files were filtered using Trim Galore! (version 0.6.10) powered by Cutadapt (version 4.2). Trimmed fastq file quality was evaluated with FastQC (version 0.12.1), and MultiQC (version 1.26) was used to generate MultiQC reports. Homo Sapiens STAR and RSEM references were built using STAR (version 2.7.11b) and RSEM (version 1.3.3), respectively, Ensembl release 112, genome assembly GRCh38 (Homo_sapiens.GRCh38.dna.primary_assembly.fa) and the following gtf annotation file: Homo_sapiens.GRCh38.112.gtf. Trimmed reads were aligned to the Homo Sapiens STAR reference with STAR (version 2.7.11b) and alignment log files were compiled with MultiQC (version 1.26). Aligned reads were assessed for strandedness using RSeQC infer_experiment.py (version 5.0.4), gene body coverage using RSeQC geneBody_coverage.py (version 5.0.4), and distribution of RNA features using RSeQC read_distribution.py (version 5.0.4). Aligned reads from all samples were quantified using RSEM (version 1.3.3), with strandedness set to none and RSEM count log files were compiled with MultiQC (version 1.26). To create a set of rRNA-removed quantification data, Ensembl IDs mapping to ribosomal RNA (rRNA) were removed from the RSEM gene count data. Both the original set of RSEM quantification data and the rRNA-removed data were subject to differential gene expression analysis as follows. A runsheet was generated with dp_tools (version 1.3.8) and the runsheet and quantification data were imported to R (version 4.4.2) with tximport (version 1.34.0) and normalized with DESeq2 (version 1.46.0) median of ratios method. Normalized gene counts were subject to differential expression analysis. Differential expression analysis was performed in R (version 4.4.2) using DESeq2 (version 1.46.0); all groups were compared pairwise using the Wald test and the likelihood ratio test was used to generate the F statistic p-value. Gene annotations were assigned using the custom annotation tables generated in-house as detailed in GL-DPPD-7110-A (<a href=\"https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable-A_1.1.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A.md\" target=\"_\" >https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable-A_1.1.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A.md</a>), with STRINGdb (version 2.16.4), PANTHER.db (version 1.0.12), and org.Hs.eg.db (version 3.19.1).","name":"GeneLab RNAseq data processing protocol","parameters":[{"@id":"#140227422491216","parameterName":{"@id":"W9tes96RPBkIuuA0eNexPPFOXF4m5lLPOIBTnVsM","annotationValue":"trimmed sequence data","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0002569","termSource":"OBI"}},{"@id":"#140227422491168","parameterName":{"@id":"s2HskUjUzSQmahcRYDfv1xBVjYScvewE8EO773hd","annotationValue":"Trimmed Sequence Data/Trimming Reports","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422494336","parameterName":{"@id":"Paz4Pjo6sV6E66RG3a9xuZDjKKpk7ywwq3VwmAwn","annotationValue":"Trimmed Sequence Data/MultiQC Reports","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422491648","parameterName":{"@id":"lqpWRJZ7O2rPhdA4uPNANCqeFig4cQhw07RfB2f5","annotationValue":"aligned sequence data","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0002580","termSource":"OBI"}},{"@id":"#140227422494144","parameterName":{"@id":"SPhWEr1D3r2VJ9OKO5b94MEY02Ipod6UwkLtQaRk","annotationValue":"Aligned Sequence Data/Unmapped Reads","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422492704","parameterName":{"@id":"3AwnKZQDUPRaMntinM9bvBrGHpnb8qc6GaaZRIQh","annotationValue":"Aligned Sequence Data/Alignment Logs","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422494288","parameterName":{"@id":"ib7AEGeVUoAruCpz91Qn6GXdO3E5d8k0DSeT2EQR","annotationValue":"Aligned Sequence Data/MultiQC Reports","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422491792","parameterName":{"@id":"QcbiokEiY9OO2PO8IXfNhCwNUeMq4JM2ugyguLO3","annotationValue":"RSeQC/MultiQC Reports","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422494480","parameterName":{"@id":"CzV3xNixKDCRnULWE97WbT9v456v77sgf4dPZG0Y","annotationValue":"Raw Counts Data","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422491936","parameterName":{"@id":"WXhsQG8JLZ5oK8kUYbPxGSfXi520yz8A4dEHcBKs","annotationValue":"Raw Counts Tables","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422492368","parameterName":{"@id":"w31o7Q91WYOVYIwRCqOCvdUs4H84WG2M5y8Qgu1N","annotationValue":"Raw Counts Data/MultiQC Reports","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422492800","parameterName":{"@id":"Lns3jxVPjBQ2dUuFO7KyPzSqST1hCHcuYbzwld4i","annotationValue":"Normalized Counts Data","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#140227422492128","parameterName":{"@id":"c4UoiNar4sA4ty0VawH08rhaf4AR7F8drWBP91tt","annotationValue":"differential expression analysis data","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0002584","termSource":"OBI"}}],"protocolType":{"@id":"gHRCpX8ZCZrvMb1jv0PpSihU4DurynDUUnkOSfet","annotationValue":"data transformation","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0200000","termSource":"OBI"},"uri":"","version":""}],"publicReleaseDate":"19-Mar-2025","publications":[{"authorList":"Hwang H, Rampoldi A, Forghani P, Li D, Fite J, Boland G, Maher K, Xu C.","comments":[],"doi":"10.1038/s41526-023-00336-6","pubMedID":"38071377","status":{"@id":"LmdAvi3HYaAIgsfwpY1sWHGrDR5sHm3luQJDg1Ay","annotationValue":"published","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0001796","termSource":"EFO"},"title":"Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres."}],"studyDesignDescriptors":[],"submissionDate":"20-Mar-2023","title":"Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres","unitCategories":[{"@id":"0egjOe4tupLenTJ2RAdBhRffOpl8xZWv0FRLbF9W","annotationValue":"day","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000033","termSource":""},{"@id":"ixhZXi79C7bGPbpBgn0nhYS3WcKUHFxvfrXat1ju","annotationValue":"degree Celsius","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000027","termSource":""},{"@id":"s4mQc0cEaxmkRxH4swt1u3Kwmsdo3hiNE43z9pX9","annotationValue":"hour","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000032","termSource":""}]}],"version":3}},"success":true}
