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Megan McArthur, and Mark Vande Hei who performed the experiments on the ISSNL. We also acknowledge members from BioServe Space Technologies for technical and logistical support associated with the launch, namely Matthew Vellone, Shankini Doraisingam, Mark Rupert, Stefanie Countryman, and Sheila Murphy. We acknowledge project management assistance of Kristin Kopperud and Marc Giulianotti from CASIS. Finally, we acknowledge the clinical muscle RNA samples from the University of Kentucky Center for Muscle Biology. Figures 1 and 2, and the graphical abstract were created with Biorender.com."},{"name":"DOI","value":"10.26030/0rx9-xz98"},{"name":"Data Source Accession","value":"GSE238215"},{"name":"Data Source Link","value":"https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE238215"},{"name":"Experiment Platform","value":"Tissue Chips in Space"},{"name":"Flight Program","value":"International Space Station (ISS)"},{"name":"Funding","value":"This work was supported in part by grants to N.F.H. from the US National Institutes of Health (R01 HL142718, R41 HL170875, and R21 HL172096-01), the US Department of Veterans Affairs (1I01BX004259, 5I01RX001222, and 1I21RX004898), the National Science Foundation (1829534 and 2227614), the Center for the Advancement of Science in Space (CASIS, 80JSC018M0005), and the American Heart Association (20IPA35360085 and 20IPA35310731) and by grants to T.A.R. from the US National Institutes of Health (R01 AG068667 and P01 AG036695) and the US Department of Veterans Affairs (I01BX002324 and I01RX001222). N.F.H. is a recipient of a Research Career Scientist award (IK6 BX006309) from the Department of Veterans Affairs."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":"Kennedy Space Center (KSC)"},{"name":"Mission End","value":"12/15/2021"},{"name":"Mission Link","value":"https://osdr.nasa.gov/bio/repo/data/missions/NG-16"},{"name":"Mission Name","value":"NG-16"},{"name":"Mission Start","value":"08/10/2021"},{"name":"Project Identifier","value":"CM"},{"name":"Project Link","value":""},{"name":"Project Title","value":""},{"name":"Project Type","value":"Spaceflight Study"},{"name":"Space Program","value":"NASA, NIH"},{"name":"Study Grant Number","value":""}],"description":"Microgravity has been shown to lead to both muscle atrophy and impaired muscle regeneration. The purpose was to study the efficacy of microgravity to model impaired muscle regeneration in an engineered muscle platform and then to demonstrate the feasibility of performing drug screening in this model. Engineered human muscle was launched to the International Space Station National Laboratory, where the effect of microgravity exposure for 7\u00a0days was examined by transcriptomics and proteomics approaches. Gene set enrichment analysis of engineered muscle cultured in microgravity, compared to normal gravity conditions, highlighted a metabolic shift toward lipid and fatty acid metabolism, along with increased apoptotic gene expression. The addition of pro-regenerative drugs, insulin-like growth factor-1 (IGF-1) and a 15-hydroxyprostaglandin dehydrogenase inhibitor (15-PGDH-i), partially inhibited the effects of microgravity. In summary, microgravity mimics aspects of impaired myogenesis, and the addition of these drugs could partially inhibit the effects induced by microgravity. Only data for myoblast samples are reported here. 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A runsheet was generated with dp_tools (version 1.3.8) and the runsheet and quantification data were imported to R (version 4.4.2) with tximport (version 1.34.0) and normalized with DESeq2 (version 1.46.0) median of ratios method. Normalized gene counts were subject to differential expression analysis. Differential expression analysis was performed in R (version 4.4.2) using DESeq2 (version 1.46.0); all groups were compared pairwise using the Wald test and the likelihood ratio test was used to generate the F statistic p-value. 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