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We thank the Virgin Galactic personnel who supported experiment operations at Spaceport America launch facilities, in particular Maya Naphade and the other members of the flight crew. We appreciate support and services from members of UF Interdisciplinary Center for Biotechnology Research (ICBR) in cores of Bioinformatics (RRID:SCR_019120), NextGen DNA sequencing (RRID:SCR_019152) and Gene Expression and Genotyping (RRID:SCR_019145), and the support of all members of the UF Space Plants Laboratory."},{"name":"DOI","value":"10.26030/8ane-wq52"},{"name":"Data Source Accession","value":"PRJNA984625"},{"name":"Data Source Link","value":"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA984625"},{"name":"Experiment Platform","value":"Kennedy Space Center Fixation Tubes (KFTs)"},{"name":"Flight Program","value":"Virgin Galactic SpaceShipTwo"},{"name":"Funding","value":"NASA Flight Opportunities Program (FOP) grant #80NSSC18K1294"},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":"Kennedy Space Center (KSC)"},{"name":"Mission End","value":"2021-06-11"},{"name":"Mission Link","value":"https://osdr.nasa.gov/bio/repo/data/missions/Unity 22"},{"name":"Mission Name","value":"Unity 22"},{"name":"Mission Start","value":"2021-06-11"},{"name":"Project Identifier","value":"UF-HT-KFT-01"},{"name":"Project Link","value":""},{"name":"Project Title","value":""},{"name":"Project Type","value":"High Altitude Study"},{"name":"Space Program","value":"NASA"},{"name":"Study Grant Number","value":""}],"description":"The Virgin Galactic Unity 22 mission conducted the first astronaut-manipulated suborbital spaceflight experiment. 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In addition, gene families largely unaffected in orbital spaceflight were diversely regulated in suborbital flight, including stress-responsive transcription factors. 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In short, raw fastq files were filtered using Trim Galore! (version 0.6.10) powered by Cutadapt (version 4.2). Trimmed fastq file quality was evaluated with FastQC (version 0.12.1), and MultiQC (version 1.26) was used to generate MultiQC reports. Arabidopsis Thaliana STAR and RSEM references were built using STAR (version 2.7.11b) and RSEM (version 1.3.3), respectively, Ensembl Plants release 59, genome assembly TAIR10 (Arabidopsis_thaliana.TAIR10.dna.toplevel.fa) and the following gtf annotation file: Arabidopsis_thaliana.TAIR10.59.gtf. Trimmed reads were aligned to the Arabidopsis Thaliana STAR reference with STAR (version 2.7.11b) and alignment log files were compiled with MultiQC (version 1.26). Aligned reads were assessed for strandedness using RSeQC infer_experiment.py (version 5.0.4), gene body coverage using RSeQC geneBody_coverage.py (version 5.0.4), inner distance was assessed using RSeQC inner_distance.py (version 5.0.4), and distribution of RNA features using RSeQC read_distribution.py (version 5.0.4). Aligned reads from all samples were quantified using RSEM (version 1.3.3), with strandedness set to reverse and RSEM count log files were compiled with MultiQC (version 1.26). A runsheet was generated with dp_tools (version 1.3.8) and the runsheet and quantification data were imported to R (version 4.4.2) with tximport (version 1.34.0) to generate the RSEM raw counts data. 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