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The hopTum-l strain carries a dominant point mutation in the human JAK-kinase homolog, hopscotch (Luo et al., 1995). This gain-of-function mutation confers constitutive JAK-STAT signaling. High rates of hematopoietic proliferation in larval stages result in small, melanized tumors that can be scored through the adult stages. In this strain, the misshapen (msn)- promoter directs GAL4 expression to lamellocytes (Tokusumi et al., 2009), which drives the expression of the UAS-mCD8-GFP transgene to visualize lamellocytes that make up the bulk of the tumors. Extensive pre-flight testing was done to estimate the diet volume and insect numbers for optimal growth during the 33-day flight period (April 2\u2013May 5, 2018). Six conditions (two fly-only cultures, four fly/wasp co-cultures) were distributed between six Vented Fly Boxes (VFBs). The fly-only cultures were reared in polystyrene vials containing 12 ml +/- 1 ml fly food, while the co-culture vials contained 10 ml +/- 1 ml fly food. 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