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grant (ANR-17-CE13-0004-01). Montpellier GenomiX acknowledges financial support from the France G\u00e9nomique National infrastructure, funded as part of \u2018Investissement d\u2019Avenir\u2019 programme at the Agence Nationale de la Recherche (contract ANR-10-INBS-09)."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":""},{"name":"Mission End","value":""},{"name":"Mission Link","value":""},{"name":"Mission Name","value":""},{"name":"Mission Start","value":""},{"name":"Project Identifier","value":"Simulated_Environmental_Factors"},{"name":"Project Link","value":""},{"name":"Project Title","value":""},{"name":"Project Type","value":"Ground Study"},{"name":"Space Program","value":""},{"name":"Study Grant Number","value":""}],"description":"Time-series RNAseq analysis following lateral root induction by gravistimulation. Lateral root organogenesis is a key process in the development of a plant\u2019s root system and its adaptation to the environment. During lateral root formation, an early phase of cell proliferation first produces a four-cell-layered primordium, and only from this stage onwards is a root meristem-like structure, expressing root stem cell niche marker genes, being established in the developing organ. Previous studies reported that the gene regulatory network controlling lateral root formation is organized into two subnetworks whose mutual inhibition may contribute to organ patterning. PUCHI encodes an AP2/ERF transcription factor expressed early during lateral root primordium development and required for correct lateral root formation. To dissect the molecular events occurring during this early phase, we generated time-series transcriptomic datasets profiling lateral root development in puchi-1 mutants and wild types. Transcriptomic and reporter analyses revealed that meristem-related genes were expressed ectopically at early stages of lateral root formation in puchi-1 mutants. 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Approximately 400 mature root segments located between the bend and the shoot were harvested in 4-day-old seedlings at 12 h after gravistimulation to be used as a reference of root tissues devoid of developing LRPs (termed \u2018No LR\u2019 in the dataset).","name":"sample collection","parameters":[{"@id":"#140502558446544","parameterName":{"@id":"eUm691aR7an5kII9bYi7JOSQEcghOzzbVfEjAtOG","annotationValue":"Sample Preservation Method","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}},{"@id":"#140502558448896","parameterName":{"@id":"vbrzINCaNhslTc1WulmcigpF6afqM7x3H1PCAdm0","annotationValue":"Sample Storage Temperature","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}}],"protocolType":{"@id":"9AKy01Zo5kzawArYiQYhNN82sV5bfwRsBjaGG7pr","annotationValue":"sample collection","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""}],"publicReleaseDate":"25-Mar-2022","publications":[{"authorList":"Bellande K, Trinh DC, Gonzalez AA, Dubois E, Petitot AS, Lucas M, Champion A, Gantet P, Laplaze L, Guyomarc'h S.","comments":[],"doi":"10.1093/jxb/erac079","pubMedID":"35224628","status":{"@id":"cfwAwavSGugL54m5pQGCxkplxzpfut4vvBkXLzDG","annotationValue":"Published","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0001796","termSource":""},"title":"PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana."}],"studyDesignDescriptors":[{"@id":"oucQBxZpP3kzHuaCJXfP0nSzm8yg1unXFhsKCUWT","annotationValue":"Gravitropism","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D018522","termSource":"MESH"},{"@id":"xqJ7EPQkXCqew8tO4BjTmO1Yeosvb6x8iZJS4JRu","annotationValue":"RNA-Seq","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D000081246","termSource":"MESH"}],"submissionDate":"22-Mar-2022","title":"PUCHI represses early meristem formation in developing lateral roots of Arabidopsis thaliana","unitCategories":[{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""},{"@id":"0egjOe4tupLenTJ2RAdBhRffOpl8xZWv0FRLbF9W","annotationValue":"day","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000033","termSource":""},{"@id":"s4mQc0cEaxmkRxH4swt1u3Kwmsdo3hiNE43z9pX9","annotationValue":"hour","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000032","termSource":""}]}],"version":3}},"success":true}