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Root, Level Flight, replicate 1","a100001samplename":"GSM4848978","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"Level flight ","a100017factorvaluetime":"Roots of seedlings fixed during level flight ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Level Flight, replicate 2","a100001samplename":"GSM4848979","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"Level flight ","a100017factorvaluetime":"Roots of seedlings fixed during level flight ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Level Flight, replicate 3","a100001samplename":"GSM4848980","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"Level flight ","a100017factorvaluetime":"Roots of seedlings fixed during level flight ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Level Flight, replicate 4","a100001samplename":"GSM4848981","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"Level flight ","a100017factorvaluetime":"Roots of seedlings fixed during level flight ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 1, replicate 1","a100001samplename":"GSM4848982","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the first parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 1, replicate 2","a100001samplename":"GSM4848983","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the first parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 1, replicate 3","a100001samplename":"GSM4848984","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the first parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 1, replicate 4","a100001samplename":"GSM4848985","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the first parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 3, replicate 1","a100001samplename":"GSM4848986","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the third parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 3, replicate 2","a100001samplename":"GSM4848987","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the third parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 3, replicate 3","a100001samplename":"GSM4848988","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the third parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 3, replicate 4","a100001samplename":"GSM4848989","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Bulbs, Plant\" source=\"MESH\">Plant Roots </a>","a100014factorvaluealteredgravity":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018470\" title=\"A change in, or manipulation of, gravitational force. This may be a natural or artificial effect. Gravity, Artificial\" source=\"MESH\">Gravity, Altered </a>","a100017factorvaluetime":"Roots of seedlings fixed after the third parabola ","a100020characteristicsstratificationexposure":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">3-5 day</a>","a100024protocolref":"growth protocol","a100025parametervaluelightsource":"fluorescent lighting ","a100028parametervaluelightcycle":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000032\" title=\"&quot;A time unit which is equal to 3600 seconds or 60 minutes.&quot; [Wikipedia:Wikipedia] h\" source=\"UO\">24 light hour</a>","a100032parametervaluelightintensity":"80 micromole per square meter per second","a100036parametervaluequantityofgrowthmedium":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000098\" title=\"&quot;A volume unit which is equal to one thousandth of a liter or 10^[-3] L, or to 1 cubic centimeter.&quot; [NIST:NIST] ml, millilitre\" source=\"UO\">50 milliliter</a>","a100040parametervaluegrowthmedium":"0.5% Phytagel-based growth medium supplemented with: 0.5x Murashige-Skoog salts, 0.5% (w/v) sucrose, and 1x Gamborg's Vitamin Mixture ","a100043parametervaluelightregimen":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/EO_0007162\" title=\"The treatment involving continuous exposure of light to the plant.\" source=\"EFO\">continuous light regimen </a>","a100046parametervaluemeangrowthtemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">19 degree Celsius</a>","a100050parametervaluehardware":"UF growth room","a100051protocolref":"treatment protocol","a100052protocolref":"sample collection","a100053parametervaluesamplepreservationmethod":"RNALater ","a100056parametervaluesamplestoragetemperature":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000027\" title=\"&quot;A temperature unit which is equal to one kelvin degree. However, they have their zeros at different points. The centigrade scale has its zero at 273.15 K.&quot; [NIST:NIST] C\" source=\"UO\">-80 degree Celsius</a>","a100060parametervalueageatsampleharvest":"<a target=\"_blank\" href=\"http://purl.obolibrary.org/obo/UO_0000033\" title=\"&quot;A time unit which is equal to 24 hours.&quot; [Wikipedia:Wikipedia]\" source=\"UO\">10 day</a>"},{"a100000sourcename":"pCaMV35S:14-3-3k:GFP Root, Parabola 10, replicate 1","a100001samplename":"GSM4848990","a100002characteristicsorganism":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/NCBITAXON/3702\" title=\"thale-cress\" source=\"NCBITAXON\">Arabidopsis thaliana </a>","a100005characteristicsstrain":"<a target=\"_blank\" href=\"http://www.ebi.ac.uk/efo/EFO_0005156\" title=\"Wassilewskija is an Arabidopsis ecotype.\" source=\"EFO\">Wassilewskija ecotype </a>","a100008characteristicsgenotype":"pCaMV35S:14-3-3k:GFP ","a100011characteristicsorganismpart":"<a target=\"_blank\" href=\"http://purl.bioontology.org/ontology/MESH/D018517\" title=\"The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. 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Specific funding was provided by NASA Space Biology grant NNX15AB12G, NASA Flight Opportunities grant 80NSSC20K0113, and Florida Space Grant Consortium grant UCFOI-0000262765."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":"Kennedy Space Center (KSC)"},{"name":"Mission End","value":"01-Mar-2013"},{"name":"Mission Link","value":""},{"name":"Mission Name","value":"PF2013"},{"name":"Mission Start","value":"26-Feb-2013"},{"name":"Project Identifier","value":""},{"name":"Project Link","value":""},{"name":"Project Title","value":""},{"name":"Project Type","value":"Parabolic Study"},{"name":"Space Program","value":""},{"name":"Study Grant Number","value":"NASA Space Biology grant NNX15AB12G, NASA Flight Opportunities  grant  80NSSC20K0113, Florida  Space Grant Consortium grant UCFOI-0000262765"}],"description":"The increasing availability of flights on suborbital rockets creates new avenues for the study of spaceflight effects on biological systems, in particular the transitions between hypergravity and microgravity. This paper presents an initial comparison of the responses of Arabidopsis thaliana to suborbital and atmospheric parabolic flights as an important step toward characterizing these emerging suborbital platforms and their effects on biology. Transcriptomic profiling of the response of the Arabidopsis ecotype Wassilewskija (WS) to the aggregate spaceflight experiences in the Blue Origin New Shepard and Virgin Galactic SpaceShipTwo rockets revealed that the transcriptomic load induced by flight differed greatly between the two flights, yet was biologically related to traditional parabolic flight responses. The sku5 skewing mutant and 14-3-3\u03ba:GFP regulatory protein overexpression lines each showed altered intra-platform responses compared to WS in the Blue Origin and parabolic flights, respectively. An additional parabolic flight using the F-104 Starfighter showed that the response of 14-3-3\u03ba:GFP to flight was modulated in a similar manner to the WS line. Despite the differing genotypes, experimental workflows, flight profiles and platforms, alteration of gene expression remodeling central metabolic processes was commonly observed as a response to the flights. The processes included carbon and nitrogen metabolism, branched-chain amino acid degradation, and hypoxic responses. The timing and directionality of differentially-expressed genes involved in the conserved pathways differed among the platforms. 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On the flight day, KFTs were taken onboard the C-9 aircraft. Members of the group actuated the KFTs in level flight before the first parabola, and after the first, third, and tenth parabolas, fixing seedlings in RNALater","name":"treatment protocol","parameters":[{"@id":"#139923812968768","parameterName":{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"PGCRAeZJDifKUY4b2TtcTGR2iEqwo3p6PKTVbSlo","annotationValue":"sample treatment protocol","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0003809","termSource":"EFO"},"uri":"","version":""},{"@id":"#139922150661904","comments":[],"components":[],"description":"Samples were stored at 4 C, and transported on ice back to UF, where they were stored at -80 C. Samples were later removed from -80 C, and seedling roots were dissected from shoot tissues. Roots were grouped into 3-4 replicates per condition, and total RNA was extracted from roots and submitted for microarray analysis. The Affymetrix GeneChip Arabidopsis Gene (AraGene) 1.0 ST array was used to measure gene expression levels.","name":"sample collection","parameters":[{"@id":"#139919462960816","parameterName":{"@id":"eUm691aR7an5kII9bYi7JOSQEcghOzzbVfEjAtOG","annotationValue":"Sample Preservation Method","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}},{"@id":"#139919462957264","parameterName":{"@id":"vbrzINCaNhslTc1WulmcigpF6afqM7x3H1PCAdm0","annotationValue":"Sample Storage Temperature","comments":[],"termAccession":"https://genelab-data.ndc.nasa.gov/","termSource":""}},{"@id":"#139919462959664","parameterName":{"@id":"rK1SlsliL1uykhEVW8foGPSgzYo1lMv6rRe0DI9h","annotationValue":"Age at sample harvest","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}}],"protocolType":{"@id":"9AKy01Zo5kzawArYiQYhNN82sV5bfwRsBjaGG7pr","annotationValue":"sample collection","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""},{"@id":"#139922150660416","comments":[],"components":[],"description":"RNALater-fixed samples were removed from -80 C storage, and thawed overnight at 4 C. Seedlings were disentangled, and roots were dissected from shoots. Root tissues were used for RNA extraction, and shoots were restored to -80 C storage. For each experimental condition, 3-4 biological replicates, derived from roots fixed in a single KFT, were used. Excess RNALater was removed from roots, and RNA was extracted using the QIAshredder and RNAeasy kits from QIAGEN (QIAGEN Sciences, MD, USA), using the manufacturer's instructions. RNase-free DNase (QIAGEN GmbH, Hilden, Germany) was used for on-column digestion and removal of DNA","name":"RNA Extraction","parameters":[{"@id":"#139919462960624","parameterName":{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"W9Iexc2JLrJvowOT6DvyBGQ5RLVFok3Sl6LoN06y","annotationValue":"RNA Extraction","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""},{"@id":"#139919462959184","comments":[],"components":[],"description":"cDNA was synthesized from 100 ng of total RNA, and the cDNA was used as a template for in vitro transcription (IVT). The antisense RNA synthesized during the IVT reaction was used to generate sense DNA. Sense strand DNA was fragmented, biotin-labeled using the Affymetrix GeneChip WT Terminal Labeling Kit (ThermoFisher Scientific, Catalog   900670) per the manufacturer's protocol.","name":"Labeling","parameters":[{"@id":"#139919462959568","parameterName":{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"dEeweVLu5TkPmeRPDVDa1siyWNv0rpeYtdHTvFii","annotationValue":"Labeling","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""},{"@id":"#139919462960240","comments":[],"components":[],"description":"3.5 ug of sense DNA was hybridized, with rotation, onto an Affymetrix GeneChip Arabidopsis Gene 1.0 ST Array for 16 h at 45 C. Arrays were washed on a Fluidics Station 450 (ThermoFisher Scientific, Catalog   00-0079) using the Hybridization Wash and Stain Kit (Thermofisher Scientific, Catalog   900720) and the Washing Procedure FS450_0002.","name":"Nucleic Acid Hybridization","parameters":[{"@id":"#139923803299504","parameterName":{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"Ds5U52KXWGMCUj85Xzb7xXlQahYabetbZxYQOx7G","annotationValue":"Nucleic Acid Hybridization","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""},{"@id":"#139919462957648","comments":[],"components":[],"description":"KFTs were actuated in level flight before the onset of parabolic flight, and after the first, third, and tenth parabolas. Samples were stored at 4 C, and transported on ice back to UF, where they were stored at -80 C. Samples were later removed from -80 \u00b0C, and seedling roots were dissected from shoot tissues. Roots were grouped into 3-4 replicates per condition, and total RNA was extracted from roots and submitted for microarray analysis. The Affymetrix GeneChip Arabidopsis Gene (AraGene) 1.0 ST array was used to measure gene expression levels. The transcriptomic data from the samples fixed in parabolic flight were then compared to those fixed during level flight in order to identify differentially-expressed genes associated with parabolic flight patterns.","name":"Data Collection","parameters":[{"@id":"#139923803295856","parameterName":{"@id":"3Hq6ViAPdhnUQVpmPN4g5qe1Sc2CIbQALaTc3jdF","annotationValue":"","comments":[],"termAccession":"","termSource":""}}],"protocolType":{"@id":"aDEizhRz0daWSB2Gps2fM88el5h72edi83NoKkwU","annotationValue":"Data Collection","comments":[],"termAccession":"","termSource":"EFO"},"uri":"","version":""},{"@id":"#139919462958416","comments":[],"components":[],"description":"Data were processed as described in GL-DPPD-7114 (<a href=\"https://github.com/nasa/GeneLab_Data_Processing/blob/master/Microarray/Affymetrix/Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md\" target=\"_\" >https://github.com/nasa/GeneLab_Data_Processing/blob/master/Microarray/Affymetrix/Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md</a>) using NF_MAAffymetrix version 1.0.1 (GitHub link coming soon).  In short, a RunSheet containing raw data file location and processing metadata from the study's *ISA.zip file was generated using dp_tools (version 1.3.2). The raw array data files were loaded into R (version 4.1.3) using oligo (version 1.58.0). Raw data quality assurance density plot, pseudo images, MA plots, and boxplots were generated using oligo (version 1.58.0). The raw probe level intensity data was background corrected and normalized across arrays via the oligo (version 1.58.0) quantile method. Normalized probe level data quality assurance density plot, pseudo images, MA plots, and boxplots were generated using oligo (version 1.58.0).  Normalized probe level data was summarized to the probeset level using the oligo (version 1.58.0) RMA method. Ensembl gene ID mappings were retrieved for each probeset using the Plants Ensembl database ftp server (plants.ensembl.org, release 54). Differential expression analysis was performed in R (version 4.1.3) using limma (version 3.50.3); all groups were compared pairwise for each probeset to generate a moderated t-statistic and associated p- and adjusted p-value. Gene annotations were assigned for every probeset that mapped to exactly one Ensembl gene ID using the custom annotation tables generated in-house as detailed in GL-DPPD-7110 (<a href=\"https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable_1.0.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110/GL-DPPD-7110.md\" target=\"_\" >https://github.com/nasa/GeneLab_Data_Processing/blob/GL_RefAnnotTable_1.0.0/GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110/GL-DPPD-7110.md</a>), with STRINGdb (version 2.8.4), PANTHER.db (version 1.0.11), and org.At.tair.db (version 3.15.0).","name":"GeneLab microarray data processing protocol","parameters":[{"@id":"#139923803299168","parameterName":{"@id":"K118AXUyIEG1ZYS51uguXcjP8ntWEkDmw8vHFKwv","annotationValue":"Raw Intensities Table","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#139923803299216","parameterName":{"@id":"RpH2RFEgfhHH505JAZKmbs1qSQxqTEWunuvVxI6T","annotationValue":"Normalized Expression Table","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""}},{"@id":"#139924626551904","parameterName":{"@id":"c4UoiNar4sA4ty0VawH08rhaf4AR7F8drWBP91tt","annotationValue":"differential expression analysis data","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0002584","termSource":"OBI"}}],"protocolType":{"@id":"gHRCpX8ZCZrvMb1jv0PpSihU4DurynDUUnkOSfet","annotationValue":"data transformation","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0200000","termSource":"OBI"},"uri":"","version":""}],"publicReleaseDate":"03-Apr-2021","publications":[{"authorList":"Califar Brandon, Zupanska Agata, Callaham Jordan A., Bamsey Matthew T., Graham Thomas, Paul Anna-Lisa, Ferl Robert J.","comments":[],"doi":"10.2478/gsr-2021-0002","pubMedID":"","status":{"@id":"LmdAvi3HYaAIgsfwpY1sWHGrDR5sHm3luQJDg1Ay","annotationValue":"published","comments":[],"termAccession":"http://www.ebi.ac.uk/efo/EFO_0001796","termSource":"EFO"},"title":"Shared Metabolic Remodeling Processes Characterize the Transcriptome of Arabidopsis thaliana within Various Suborbital Flight Environments"}],"studyDesignDescriptors":[{"@id":"SpmHfVYVbiMXL7SQfM2mOpEKy1nuh3YMIZCGP40e","annotationValue":"transcription profiling assay","comments":[],"termAccession":"http://purl.obolibrary.org/obo/OBI_0000424","termSource":"OBI"},{"@id":"k8rppZHuvd3QhkjZ7dYmbh6mJcuFjH5YjOrZK3MB","annotationValue":"Weightlessness","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D014893","termSource":"MESH"}],"submissionDate":"22-Oct-2020","title":"Arabidopsis thaliana in altered gravity: the 14-3-3\u03ba:GFP overexpression line in C-9 parabolic flight, 2013","unitCategories":[{"@id":"0egjOe4tupLenTJ2RAdBhRffOpl8xZWv0FRLbF9W","annotationValue":"day","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000033","termSource":""},{"@id":"ixhZXi79C7bGPbpBgn0nhYS3WcKUHFxvfrXat1ju","annotationValue":"degree Celsius","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000027","termSource":""},{"@id":"s4mQc0cEaxmkRxH4swt1u3Kwmsdo3hiNE43z9pX9","annotationValue":"hour","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000032","termSource":""},{"@id":"yiWpT2is4TWDOOWFqUPoSDZD1BaCkAfgP2yL338f","annotationValue":"micromole per square meter per second","comments":[],"termAccession":"https://osdr.nasa.gov/","termSource":""},{"@id":"u5hUzYE0N0xDfj0iUQCW3tdbfFRfHT1ccYPDERfY","annotationValue":"milliliter","comments":[],"termAccession":"http://purl.obolibrary.org/obo/UO_0000098","termSource":""}]}],"version":3}},"success":true}