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Accession","value":""},{"name":"Data Source Link","value":""},{"name":"Experiment Platform","value":"BRIC-PDFU (Biological Research In Canisters - Petri Dish Fixation Unit)"},{"name":"Flight Program","value":"International Space Station (ISS)"},{"name":"Funding","value":"The Center is partially supported by NCI Cancer Center Support Grant #P30 CA91842 to the Siteman Cancer Center and by ICTS/CTSA Grant# UL1 TR000448 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research. This publication is solely the responsibility of the authors and does not necessarily represent the official view of NCRR or NIH. And finally, this research was partially funded by NASA (#NNX13AM48G to SEW and DRL) with supplemental funding provided by NASA GeneLab."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":"Kennedy Space Center (KSC)"},{"name":"Mission End","value":"02/11/2015"},{"name":"Mission Link","value":"https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-5"},{"name":"Mission Name","value":"SpaceX-5"},{"name":"Mission Start","value":"01/10/2015"},{"name":"Project Identifier","value":"BRIC-20"},{"name":"Project Link","value":"https://lsda.jsc.nasa.gov/scripts/experiment/exper.aspx?exp_index=13836"},{"name":"Project Title","value":"Proteomics analysis of Arabidopsis seedlings in microgravity"},{"name":"Project Type","value":"Spaceflight Study"},{"name":"Space Program","value":"NASA"},{"name":"Study Funding Agency","value":"NASA Space Biology Program Office, SLPSRA (Space Life Sciences and Physical Sciences Research and Applications) Division"},{"name":"Study Grant Number","value":"NNX13AM48G"}],"description":"On Earth plants are constantly exposed to a gravitational field of 1G. 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The BRICs were maintained at 4C until spaceflight to ensure seed germination in microgravity. After 3 days of germination and growth, the seedlings were fixed by injecting RNAlater into the chamber. They were kept at ambient temperature for 12 hours followed by freezing at -80C. An additional 22 plates were used as ground controls. After the spaceflight, tissue from five plates was pooled to make each of three replicates. Both membrane and soluble proteins were extracted from the pooled seedlings. 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