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The Dragon capsule unberthed at 13:26 UTC (Co-ordinated Universal Time) on May 18, 2014 and re-entry into the Earth\u2019s atmosphere occurred the same day at 19:05 UTC. Following retrieval of the Dragon capsule off the coast of Mexico, the VFB was offloaded at Long Beach Port, California and transported by car to La Jolla, California in a climate-controlled vehicle. The VFB was oriented such that the g\u2010load was directed down into the food supply located at the bottom of the vials. This orientation was maintained throughout the entirety of the mission, from point of transfer at the lab at NASA Kennedy Space Center through stowage on the Dragon capsule, and again from Dragon un-berthing to opening of the VFB in the lab at SBP Medical Discovery Institute in La Jolla, California. 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Aligned reads were assessed for strandedness using RSeQC infer_experiment.py (version 5.0.4), gene body coverage using RSeQC geneBody_coverage.py (version 5.0.4), inner distance was assessed using RSeQC inner_distance.py (version 5.0.4), and distribution of RNA features using RSeQC read_distribution.py (version 5.0.4). Aligned reads from all samples were quantified using RSEM (version 1.3.3), with strandedness set to reverse and RSEM count log files were compiled with MultiQC (version 1.26). To create a set of rRNA-removed quantification data, Ensembl IDs mapping to ribosomal RNA (rRNA) were removed from the RSEM gene count data. Both the original set of RSEM quantification data and the rRNA-removed data were subject to differential gene expression analysis as follows. A runsheet was generated with dp_tools (version 1.3.8) and the runsheet and quantification data were imported to R (version 4.4.2) with tximport (version 1.34.0) and normalized with DESeq2 (version 1.46.0) median of ratios method. Normalized gene counts were subject to differential expression analysis. Differential expression analysis was performed in R (version 4.4.2) using DESeq2 (version 1.46.0); all groups were compared pairwise using the Wald test and the likelihood ratio test was used to generate the F statistic p-value. 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