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SEEDLING GROWTH experiment was supported by the Spanish 'Plan Estatal de Investigacion Cientifica y Tecnica e Innovacion' of the Ministry of Economy, Industry and Competitiveness (Grant numbers AYA2012-33982 and ESP2015-64323-R to FJM, cofounded by ERDF). Funding for the transcriptomic sequencing analysis performed by NASA's GeneLab Project was provided by NASA Space Biology Program Office, Biological and Physical Sciences Division of the Science Mission Directorate."},{"name":"Identifiers","value":""},{"name":"Managing NASA Center","value":""},{"name":"Mission End","value":"03/26/2013"},{"name":"Mission Link","value":"https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-2"},{"name":"Mission Name","value":"SpaceX-2"},{"name":"Mission Start","value":"03/01/2013"},{"name":"Project Identifier","value":"Seedling Growth-1"},{"name":"Project Link","value":"https://lsda.jsc.nasa.gov/Experiment/exper/1933"},{"name":"Project Title","value":"Novel Explorations Into the Interactions Between Light and Gravity Sensing in Plants - Seedling Growth-1"},{"name":"Project Type","value":"Spaceflight Study"},{"name":"Space Program","value":"NASA, ESA"},{"name":"Study Grant Number","value":""}],"description":"Traveling to nearby extraterrestrial objects having a reduced gravity level (partial gravity) compared to Earth's gravity is becoming a realistic objective for space agencies. The use of plants as part of life support systems will require a better understanding of the interactions among plant growth responses including tropisms, under partial gravity conditions. Here, we present results from the Seedling Growth space experiments on the ISS, to complement the previously released GLDS-251 dataset including seeds of Arabidopsis thaliana wildtype plants. Seeds were germinated, and seedlings grew for six days under different gravity levels, namely micro-g, several intermediate partial-g levels, and 1g, and were subjected to irradiation with blue light for the last 48 hours. RNA was extracted was obtained for 20 wildtype samples for subsequent RNAseq analysis in GLDS-251, here we add 36 samples from similarly exposed PhyA and PhyB mutants.","factors":[{"@id":"kLiWFCEDa9Rq1kskvTpT8zZ1KRoJgq8IerbpynLL","comments":[],"factorName":"Genotype","factorType":{"@id":"c74v6NZk3hqwsKxwVYs5ZQJoDyALsSQzwlGGfuxB","annotationValue":"Genotype","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D005838","termSource":"MESH"}},{"@id":"rrAHixexWAdqOKNaj15Ezk66kWZSMcprcBRZfg1k","comments":[],"factorName":"Spaceflight","factorType":{"@id":"XJ2x9G5FYOhyGGbYDO0xkevuzcPxPnzdf1nFa9fv","annotationValue":"Space Flight","comments":[],"termAccession":"http://purl.bioontology.org/ontology/MESH/D013026","termSource":"MESH"}},{"@id":"XVwaJlTIF2q47sfrQq99a9PcHaoTfif10Dabpwg2","comments":[],"factorName":"Altered Gravity","factorType":{"@id":"PDmY1sKTXFdQ1hZVG6o5TrZ6BxH0kq1JMb1RuJZC","annotationValue":"Gravity, 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The angular speed to generate each nominal fractional gravity level was calculated for the cassette in the center of the Experimental Container. In the case of the 1.0g cassettes, the value was calculated for the fifth cassette in order to prevent values higher than Earth nominal gravity. Seedlings were illuminated under white light (30-40 micromoles per meter squared per second) for 96 h, followed by 48 hours of unidirectional photostimulation with blue light. Some samples were exposed to a short red light treatment also (but the main illumination stimuli is still blue light). Light sources were LEDs. Light Treatment 1: Seedlings were illuminated under white light (30-40 micromoles per square meter per second) for 96 h, followed by 48 hours of unidirectional photostimulation with blue light. Light sources were LEDs. 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Some of the factors that lead to variations in the absorbed dose rate in orbit are ISS altitude, period during the solar cycle, and location of the sample\u2014the ISS module and the specific location within that module. Shielding effects from the biological payload container or habitat have not been taken into account. The measured absorbed dose can vary based on the dosimetry instrument(s) used. Radiation monitoring on the ISS is not standardized across modules; each international partner has its own suite of instruments, with differing technologies, sensitivities and analysis methodologies, including criteria for distinguishing between lightly ionizing particles (mainly protons) and highly ionizing particles (mainly galactic cosmic ray nuclei). Both active and passive dosimeters are used. Active dosimeters are powered and data are read out and stored and/or telemetered to ground. Some active dosimeters provide additional levels of detail such as time resolution, energy deposition and particle identification. Passive dosimeters are unpowered, record constantly and are read out upon return to the ground. The dose in the metadata is the dose recorded by the radiation detector closest to the sample. In most cases that detector will be in the same ISS module; in cases where data from a detector in the same module are not available, the dose is extrapolated using data from periods where data are available from detectors in multiple modules, including the module containing the payload. The duration of the experiment is defined as the time in space during which the specimen was \u201cliving\u201d, including the time on the delivery vehicle (e.g. SpaceX Dragon). Once the specimen was frozen or fixed, the experiment was considered stopped. Details of ISS dosimetry and a table with doses for all GeneLab ISS experiment ISS datasets are available at <a href=\"https://genelab.nasa.gov/environmental/radiation.\" target=\"_\" >https://genelab.nasa.gov/environmental/radiation.</a> The first set of seedlings (SG1) were uploaded on SpaceX CRS-2 (March 2013) and returned on CRS-3 (May 2014), and the second set of seedlings (SG2) were carried to the ISS on SpaceX CRS-4 (September 2014) and returned on CRS-5 (February 2015). Dates from R. Herranz (priv. comm.).   The total mission duration of SG1 was ~443 days and SG2 was ~147 days. Seeds were dormant at launch, then germinated and grown for six days, then harvested and frozen. Absorbed radiation dose and dose rate reported are from the germination to harvest. 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